Difference between revisions of "Part:BBa K1539001:Experience"
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 21:04, 1 November 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1539001
BBa_K1539001 was created using devices BBa_K1539021 and BBa_K1539055 to insert a relatively high expressing promoter and a high efficiency RBS site in front of mCherry from BBa_J0650. Expression of mCherry was quantified by flow cytometry using a FACS AriaIII. Relative to cells transfected with an mCherry plasmid without a promoter or RBS site, YYY% of our cells expressed mCherry and the median intensity was 4X brighter than a low promoter/weak RBS combination (see part [1]). Our raw data is summarized below:
"High Expression Combination" - Primers PH1/RH1:
Files:140821_DMC_mCherry_PH2-RH2.pdf
Negative Control:
File:140821 DMC mCherry Promoterless.pdf
User Reviews
UNIQ130846dc7445e692-partinfo-00000000-QINU UNIQ130846dc7445e692-partinfo-00000001-QINU