Difference between revisions of "Part:BBa K1539001:Experience"
Stassoulas3 (Talk | contribs) (→Applications of BBa_K1539001) |
Stassoulas3 (Talk | contribs) (→Applications of BBa_K1539001) |
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"High Expression Combination" - Primers PH1/RH1: | "High Expression Combination" - Primers PH1/RH1: | ||
| − | + | [[Files:140821_DMC_mCherry_PH2-RH2.pdf]] | |
Negative Control: | Negative Control: | ||
| + | |||
| + | [[Files:140821_DMC_mCherry_Promoterless.pdf]] | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 21:04, 1 November 2014
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K1539001
BBa_K1539001 was created using devices BBa_K1539021 and BBa_K1539055 to insert a relatively high expressing promoter and a high efficiency RBS site in front of mCherry from BBa_J0650. Expression of mCherry was quantified by flow cytometry using a FACS AriaIII. Relative to cells transfected with an mCherry plasmid without a promoter or RBS site, YYY% of our cells expressed mCherry and the median intensity was 4X brighter than a low promoter/weak RBS combination (see part [1]). Our raw data is summarized below:
"High Expression Combination" - Primers PH1/RH1:
Files:140821_DMC_mCherry_PH2-RH2.pdf
Negative Control:
Files:140821_DMC_mCherry_Promoterless.pdf
User Reviews
UNIQ2e63bc7f03bfb61d-partinfo-00000000-QINU UNIQ2e63bc7f03bfb61d-partinfo-00000001-QINU