Difference between revisions of "Part:BBa K1413021"

Latest revision as of 21:01, 1 November 2014

bphR2 mutated

bphR2 gene, from Pseudomonas pseudoalcaligenes KF707, encodes for a regulatory protein (bphR2) which can detect polychlorinated biphenyls (PCBs). It is involved in the positive regulation of bphr1 gene. In the absence of biphenyl, small amounts of bphR2 protein binds to bphR2 operator to repress bphR2 transcription and activate bphR1 weakly. When there is biphenyl in the media, bphR2 protein binds to bphR1 to activate strongly its transcription. This allows to initiate the degradation of biphenyl.

Our aim was to finish and characterize the part of 2013 Paris Saclay's team who wanted to do a PCB biosensor with the system bphR2/bphR1. We used bphR2 in our biosensor project of PCBs. We have a fluorescence system with RFP (BBa_E1010) to detect compound.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 475
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 259

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 __NOTOC__
 <partinfo>BBa_K1413021 short</partinfo>
-bphR2 gene, from Pseudomonas pseudoalcaligenes KF707, encodes for a regulatory protein (bphR2) which can detect polychlorinated biphenyls (PCBs). This gene regulates negatively bphR1 promoter involved in the transcription of biphenyl-catabolic genes (bph), degrading PCBs.
+bphR2 gene, from Pseudomonas pseudoalcaligenes KF707, encodes for a regulatory protein (bphR2) which can detect polychlorinated biphenyls (PCBs). It is involved in the positive regulation of bphr1 gene. In the absence of biphenyl, small amounts of bphR2 protein binds to bphR2 operator to repress bphR2 transcription and activate bphR1 weakly. When there is biphenyl in the media, bphR2 protein binds to bphR1 to activate strongly its transcription. This allows to initiate the degradation of biphenyl.
-In absence of PCB, bphR2 is bound to the bphR1 promoter. When compound  diffuses into the cell, it binds to  protein allowing a conformational change. BphR2 is release from the promoter that its allows the transcription of bph genes and therefore the degration of PCBs.
-Our aim was to finish and characterize the part of 2013 Paris Saclay's team. We used bphR2 in our biosensor project of PCBs. Instead of the genes of degradation, we have RFP (BBa_E1010) to detect 2 hydroxy-3',4' biphenyl.
+Our aim was to finish and characterize the part of 2013 Paris Saclay's team who wanted to do a PCB biosensor with the system bphR2/bphR1.
+We used bphR2 in our biosensor project of PCBs. We have a fluorescence system  with RFP (BBa_E1010) to detect compound.
 <!-- Add more about the biology of this part here