Difference between revisions of "Part:BBa K1539002:Experience"
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"Low Expression Combination" - Primers PL1/RL1: | "Low Expression Combination" - Primers PL1/RL1: | ||
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| + | [[File:140821_DMC_mCherry_PL1-RL1.pdf|200px|thumb|left|Raw data for PL1-RL1 driving mCherry expression measured on FACS AriaIII.]] | ||
[[File:140821_DMC_mCherry_PL1-RL1.pdf]] | [[File:140821_DMC_mCherry_PL1-RL1.pdf]] | ||
Revision as of 20:38, 1 November 2014
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Applications of BBa_K1539002
BBa_K1539001 was created using devices BBa_K1539034 and BBa_K1539089 to insert relatively high expressing promoters and high efficiency RBS sites in front of mCherry from BBa_J0650. Expression of mCherry was quantified by flow cytometry using a FACS AriaIII. Relative to cells transfected with an mCherry plasmid without a promoter or RBS site, 91.6% of our cells expressed mCherry and the median intensity was 4X dimmer than a high promoter/high RBS combination (see part [1]). Our raw data is summarized below:
"Low Expression Combination" - Primers PL1/RL1:
File:140821 DMC mCherry PL1-RL1.pdf
File:140821 DMC mCherry PL1-RL1.pdf
Negative Control:
User Reviews
UNIQe2eb5bbd947800f3-partinfo-00000000-QINU UNIQe2eb5bbd947800f3-partinfo-00000001-QINU