Difference between revisions of "Part:BBa K1391114:Design"

 
 
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__NOTOC__
 
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<partinfo>BBa_K1391114 short</partinfo>
 
<partinfo>BBa_K1391114 short</partinfo>
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===Design Notes===
 
===Design Notes===
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None
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This part was created using a single pot LR reaction. The promoter is flanked by B4 and P1 sites and the gene is flanked by P1 and B2 sites. Either of these can be extracted using a BP reaction. This part adheres to RFC 65 for recombination based cloning of mammalian parts.
  
  

Latest revision as of 20:37, 1 November 2014

pEXPR hEF1a: LilrB_TCS_Gal4VP16


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 5437
    Illegal EcoRI site found at 6117
    Illegal EcoRI site found at 8034
    Illegal EcoRI site found at 8075
    Illegal XbaI site found at 6043
    Illegal PstI site found at 2677
    Illegal PstI site found at 3656
    Illegal PstI site found at 3939
    Illegal PstI site found at 4564
    Illegal PstI site found at 5069
    Illegal PstI site found at 7063
    Illegal PstI site found at 7085
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 5437
    Illegal EcoRI site found at 6117
    Illegal EcoRI site found at 8034
    Illegal EcoRI site found at 8075
    Illegal NheI site found at 3210
    Illegal PstI site found at 2677
    Illegal PstI site found at 3656
    Illegal PstI site found at 3939
    Illegal PstI site found at 4564
    Illegal PstI site found at 5069
    Illegal PstI site found at 7063
    Illegal PstI site found at 7085
    Illegal NotI site found at 8917
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 5437
    Illegal EcoRI site found at 6117
    Illegal EcoRI site found at 8034
    Illegal EcoRI site found at 8075
    Illegal BglII site found at 4818
    Illegal BglII site found at 8155
    Illegal BamHI site found at 2854
    Illegal BamHI site found at 3328
    Illegal BamHI site found at 5424
    Illegal BamHI site found at 5471
    Illegal BamHI site found at 5729
    Illegal BamHI site found at 9112
    Illegal XhoI site found at 3538
    Illegal XhoI site found at 5217
    Illegal XhoI site found at 7790
    Illegal XhoI site found at 8897
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 5437
    Illegal EcoRI site found at 6117
    Illegal EcoRI site found at 8034
    Illegal EcoRI site found at 8075
    Illegal XbaI site found at 6043
    Illegal PstI site found at 2677
    Illegal PstI site found at 3656
    Illegal PstI site found at 3939
    Illegal PstI site found at 4564
    Illegal PstI site found at 5069
    Illegal PstI site found at 7063
    Illegal PstI site found at 7085
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 5437
    Illegal EcoRI site found at 6117
    Illegal EcoRI site found at 8034
    Illegal EcoRI site found at 8075
    Illegal XbaI site found at 6043
    Illegal PstI site found at 2677
    Illegal PstI site found at 3656
    Illegal PstI site found at 3939
    Illegal PstI site found at 4564
    Illegal PstI site found at 5069
    Illegal PstI site found at 7063
    Illegal PstI site found at 7085
    Illegal NgoMIV site found at 2297
    Illegal NgoMIV site found at 3741
    Illegal NgoMIV site found at 4024
    Illegal NgoMIV site found at 4952
    Illegal NgoMIV site found at 6334
    Illegal NgoMIV site found at 7027
    Illegal AgeI site found at 3456
    Illegal AgeI site found at 4330
    Illegal AgeI site found at 5866
    Illegal AgeI site found at 6366
    Illegal AgeI site found at 6853
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7709
    Illegal BsaI.rc site found at 1121
    Illegal SapI site found at 38
    Illegal SapI site found at 3214
    Illegal SapI site found at 9344
    Illegal SapI.rc site found at 5706


Design Notes

This part was created using a single pot LR reaction. The promoter is flanked by B4 and P1 sites and the gene is flanked by P1 and B2 sites. Either of these can be extracted using a BP reaction. This part adheres to RFC 65 for recombination based cloning of mammalian parts.


Source

Composite: Human, Tobacco etch virus

References