Difference between revisions of "Part:BBa K1539002:Experience"
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===Applications of BBa_K1539002=== | ===Applications of BBa_K1539002=== | ||
| + | BBa_K1539001 was created using devices XXX and YYY to insert relatively high expressing promoters and high efficiency RBS sites in front of mCherry from BBa_J0650. Expression of mCherry was quantified by flow cytometry using a FACS AriaIII. Relative to cells transfected with an mCherry plasmid without a promoter or RBS site, 91.6% of our cells expressed mCherry and the median intensity was 4X brighter than a low promoter/weak RBS combination (see part [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1539002]). Our raw data is summarized below: | ||
| + | "Low Expression Combination" - Primers PL1/RL1: | ||
| − | + | file:///C:/Users/yannis/Downloads/140821_DMC_mCherry_PL1-RL1.pdf | |
| + | |||
| + | Negative Control: | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 20:32, 1 November 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1539002
BBa_K1539001 was created using devices XXX and YYY to insert relatively high expressing promoters and high efficiency RBS sites in front of mCherry from BBa_J0650. Expression of mCherry was quantified by flow cytometry using a FACS AriaIII. Relative to cells transfected with an mCherry plasmid without a promoter or RBS site, 91.6% of our cells expressed mCherry and the median intensity was 4X brighter than a low promoter/weak RBS combination (see part [1]). Our raw data is summarized below:
"Low Expression Combination" - Primers PL1/RL1:
file:///C:/Users/yannis/Downloads/140821_DMC_mCherry_PL1-RL1.pdf
Negative Control:
User Reviews
UNIQ26da45fd1d4ff526-partinfo-00000000-QINU UNIQ26da45fd1d4ff526-partinfo-00000001-QINU