Difference between revisions of "Part:BBa K1413043"

 
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<partinfo>BBa_K1413043 short</partinfo>
 
<partinfo>BBa_K1413043 short</partinfo>
  
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This part is the coding sequence for the transposase Tn10.
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This part is the coding sequence for the transposase Tn10. A cut-and-paste mechanism where the transposase allows insertion of a transposon randomly in the genome. The transposon must be surrounded by IS10 sequences.
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This transposase allows insertion of transposon randomly in the genome. The transposon must be surrounded by IS10 sequences.
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<partinfo>BBa_K1413043 parameters</partinfo>
 
<partinfo>BBa_K1413043 parameters</partinfo>
 
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===References===
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-Bender, J., & Kleckner, N. (1988). Genetic Evidence That TnlO Transposes by a Nonreplicative Mechanism, 45, 801–815
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-Crellin, P., & Chalmers, R. (2001). Protein±DNA contacts and conformational changes in the Tn 10 transpososome during assembly and activation for cleavage, 20(14).

Revision as of 19:06, 1 November 2014

Transposase Tn10

This part is the coding sequence for the transposase Tn10. A cut-and-paste mechanism where the transposase allows insertion of a transposon randomly in the genome. The transposon must be surrounded by IS10 sequences.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 244
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

-Bender, J., & Kleckner, N. (1988). Genetic Evidence That TnlO Transposes by a Nonreplicative Mechanism, 45, 801–815 -Crellin, P., & Chalmers, R. (2001). Protein±DNA contacts and conformational changes in the Tn 10 transpososome during assembly and activation for cleavage, 20(14).