Difference between revisions of "Part:BBa K1332013"

Revision as of 18:49, 1 November 2014

SmtA semi-permanent generator

This generator is capable of synthesizing SmtA (BBa_K519010). This generator consists of a mRNA circularization device (5’ side)(K1332008), SmtA (BBa_K519010) and a mRNA circularization device (3’ side)(endless translation))(K1332009). A mRNA is circular, so translation continues semi-permanently. SmtA is a metallothionein that can catch heavy metal ions such as Zn²⁺.

The efficacy of a long-chain SmtA

This experiment is now underway. The detail is in our wiki.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 545
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1332013 short</partinfo>
-This generator is capable of synthesizing SmtA ([https://parts.igem.org/Part:BBa_K519010 BBa_K519010]). This generator consists of an mRNA circularization device (5’ side), SmtA ([https://parts.igem.org/Part:BBa_K519010 BBa_K519010]) and an mRNA circularization device (3’ side). A mRNA is circular, so translation continues semi-permanently. SmtA is a metallothionein that can catch heavy metal ions such as Zn<sup>2+</sup>.
+This generator is capable of synthesizing SmtA ([https://parts.igem.org/Part:BBa_K519010 BBa_K519010]). This generator consists of a mRNA circularization device (5’ side)([https://parts.igem.org/Part:BBa_K1332008 K1332008]), SmtA ([https://parts.igem.org/Part:BBa_K519010 BBa_K519010]) and a mRNA circularization device (3’ side)(endless translation))([https://parts.igem.org/Part:BBa_K1332009 K1332009]). A mRNA is circular, so translation continues semi-permanently. SmtA is a metallothionein that can catch heavy metal ions such as Zn<sup>2+</sup>.
 <h3>The efficacy of a long-chain SmtA</h3>