Difference between revisions of "Part:BBa K1360002"
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | The XynB enzyme produced by this part can be used in pulp bleaching. It presents high activity under a wide range of temperature (55℃-99℃) and pH conditions (3-10.5). The | + | The XynB enzyme produced by this part can be used in pulp bleaching. It presents high activity under a wide range of temperature (55℃-99℃) and pH conditions (3-10.5). The optimum working condition is pH = 6.0, temperature = 65.9℃. This enzyme is expressed at high level in E.coli BL21(DE3) strain after T7 promoter. |
===Design considerations=== | ===Design considerations=== |
Revision as of 18:49, 1 November 2014
endo-1,4-beta-xylanase XynB
This part is the coding sequence for a thermostable xylanase, which specifically degrades xylan.
Usage and Biology
The XynB enzyme produced by this part can be used in pulp bleaching. It presents high activity under a wide range of temperature (55℃-99℃) and pH conditions (3-10.5). The optimum working condition is pH = 6.0, temperature = 65.9℃. This enzyme is expressed at high level in E.coli BL21(DE3) strain after T7 promoter.
Design considerations
This part is only a coding sequence without expression regulators, and is compatible with RFC10 standards, and the codon is optimized for E.coli expression.
Lab Archives
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 847
Illegal AgeI site found at 610 - 1000COMPATIBLE WITH RFC[1000]