Difference between revisions of "Part:BBa K1413041"
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It can be used in combinaison with transposon system which does not required a replicative system like in the part <a href="https://parts.igem.org/Part:BBa_K1413044">BBa_K1413044</a> . | It can be used in combinaison with transposon system which does not required a replicative system like in the part <a href="https://parts.igem.org/Part:BBa_K1413044">BBa_K1413044</a> . | ||
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| − | + | ===Sequence and Features=== | |
<partinfo>BBa_K1413041 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1413041 SequenceAndFeatures</partinfo> | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
Revision as of 18:37, 1 November 2014
Mutation of OriVR6Kgamma origin of replication (ori)
This part is an origin of replication which needs pi protein to replicate
Usage and Biology
It can be used in combinaison with transposon system which does not required a replicative system like in the part BBa_K1413044 .
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
This part requires production of pi protein from pir gene.
References
Rakowski SA, Filutowicz;Plasmid R6K Replication Control; M. Plasmid. 2013 May; 69(3)231-242