Difference between revisions of "Part:BBa K1419005"

Latest revision as of 18:33, 1 November 2014

RVG-Lamp2B-Clover

This part contains the Lamp2B coding sequence with a rabies virus glycoprotein tag and in-fusion clover (GFP) sequence for easy localization of Lamp2B C-terminal modification.

This part was used for determining the localization of C-terminal additions to Lamp2B. Clover takes the place of a DNA binding protein that will be used to target plasmids to the exosomal interior.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 119
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 153

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1419005 short</partinfo>
-This part contains the Lamp2B coding sequence with rabies virus glycoprotein tag and in-fusion clover (GFP) sequence for easy localization of Lamp2B C-terminal modification.
+This part contains the Lamp2B coding sequence with a rabies virus glycoprotein tag and in-fusion clover (GFP) sequence for easy localization of Lamp2B C-terminal modification.
+This part was used for determining the localization of C-terminal additions to Lamp2B. Clover takes the place of a DNA binding protein that will be used to target plasmids to the exosomal interior.
 <html><p><a href="https://static.igem.org/mediawiki/parts/6/68/Lamp2B2014iGEMDatasheet.JPG"><image src="https://static.igem.org/mediawiki/parts/6/68/Lamp2B2014iGEMDatasheet.JPG"; position="centre"; width="500"; height="600"; /> </a></p></html>
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
-This part was used for determining the localization of C-terminal additions to Lamp2B. Clover takes the place of a DNA binding protein that will be used to target plasmids to the exosomal interior.
 Datasheet for Part BBa_K1419005.
 <br>