Difference between revisions of "Part:BBa K577881:Experience"

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===Applications of BBa_K577881===
 
===Applications of BBa_K577881===
  
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We used the positive control BBa_K577881 to explore the appropriate concentration of L-arabinose inducing the araBAD promoter. The results are as follows.
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In 2014, SYSU-China applied BBa_K577881 into Integrated Evolution Machine (IgEM). We used the positive control BBa_K577881 to explore the appropriate concentration of L-arabinose inducing the araBAD promoter. The results are as follows.
  
 
[[File:K577881.png|400px|thumb|center|Figure 1. Fluorescence intensity changes of RFP protein induced by different concentration of L-arabinose.  As consecration of  L-arabinose raises, the fluorescence intensity increases.
 
[[File:K577881.png|400px|thumb|center|Figure 1. Fluorescence intensity changes of RFP protein induced by different concentration of L-arabinose.  As consecration of  L-arabinose raises, the fluorescence intensity increases.
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According to the result, we could use the concentration range of L-arabinose from 100μM to 50000μM get a expression gradient of RFP.
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According to the result, we could use the concentration range of L-arabinose from 100μM to 50000μM to get a expression gradient of RFP.
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===For more information please visit our wiki===
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http://2014.igem.org/Team:SYSU-China/content.html#Project/Result/RNAT
  
 
===User Reviews===
 
===User Reviews===

Revision as of 18:11, 1 November 2014


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K577881

In 2014, SYSU-China applied BBa_K577881 into Integrated Evolution Machine (IgEM). We used the positive control BBa_K577881 to explore the appropriate concentration of L-arabinose inducing the araBAD promoter. The results are as follows.

Figure 1. Fluorescence intensity changes of RFP protein induced by different concentration of L-arabinose.  As consecration of L-arabinose raises, the fluorescence intensity increases.


According to the result, we could use the concentration range of L-arabinose from 100μM to 50000μM to get a expression gradient of RFP.

For more information please visit our wiki

http://2014.igem.org/Team:SYSU-China/content.html#Project/Result/RNAT

User Reviews

UNIQ90499934120379f2-partinfo-00000000-QINU UNIQ90499934120379f2-partinfo-00000001-QINU