Difference between revisions of "Part:BBa K1413002"

Revision as of 17:31, 1 November 2014

P0 promoter-RBS SD001-sfGFP- Terminator B0015 - Pr promoter-DmpR

This part consist of a sensor of phenolic compounds based on DmpR, a transcription factor of the Ntrc family. Found in Pseudomonas sp. strain CF 600, DmpR regulates expression of the P0 promoter, which drives transcription of a single phenol degradation large operon (dmpKLMNOPQBCDEFGHI). With GFP attached to the P0 promoter, it is then possible to evaluate presence of phenol by fluorescence analysis if DmpR is expressed. This part is basically composed of [P0 promoter + sfGFP + Pr promoter + DmpR] and allows sensing of phenolic compounds thanks to a constitutively expressed DmpR that will activate expression of GFP when bound to phenol. We characterized this biosensor using phenol as the effector.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1241
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1719
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1404
Illegal BsaI.rc site found at 1945
Illegal SapI.rc site found at 211
Illegal SapI.rc site found at 2602

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 __NOTOC__
 <partinfo>BBa_K1413002 short</partinfo>
-This part consist on a sensor of phenolic compounds based on DmpR, a transcription factor of the Ntrc family. Found in Pseudomonas sp. strain CF 600, DmpR regulates expression from the Po promoter, which drives transcription from one single large operon for phenol degradation (dmpKLMNOPQBCDEFGHI). With GFP attached to P0 promoter, it is then possible to evaluate presence of phenol by fluorescence analysis, if DmpR is expressed. This part is basically composed of [P0 promoter + sfGFP + Pr promoter + DmpR] and allows sensing of phenolic compounds thanks to a constitutively expressed DmpR that will activate expression of GFP. We characterized this biosensor using phenol as effector.
+This part consist of a sensor of phenolic compounds based on DmpR, a transcription factor of the Ntrc family. Found in Pseudomonas sp. strain CF 600, DmpR regulates expression of the P0 promoter, which drives transcription of a single phenol degradation large operon  (dmpKLMNOPQBCDEFGHI). With GFP attached to the P0 promoter, it is then possible to evaluate presence of phenol by fluorescence analysis if DmpR is expressed. This part is basically composed of [P0 promoter + sfGFP + Pr promoter + DmpR] and allows sensing of phenolic compounds thanks to a constitutively expressed DmpR that will activate expression of GFP when bound to phenol. We characterized this biosensor using phenol as the effector.
 <!-- Add more about the biology of this part here