Difference between revisions of "Part:BBa K1500010"
Line 1: Line 1:
−
 
 
<partinfo>BBa_K1500010 short</partinfo>
 
<partinfo>BBa_K1500010 short</partinfo>
  
 
Contains an IPTG-sensitive promoter (BBa_J04500), with RBS.3 (BBa_00032), and reporter GFP (E0040) downstream with an additional RBS and the EmrR mutator gene. Using a fluctuation analysis mutation rate experiment, XL1Blue bacteria transformed with this construct yielded a mutation rate of 3.14E-09 mutations per cell per culture although mutation rate is dependent on growth conditions and can vary depending on experimental setup. This plasmid appears to incur a growth penalty in transformants.
 
Contains an IPTG-sensitive promoter (BBa_J04500), with RBS.3 (BBa_00032), and reporter GFP (E0040) downstream with an additional RBS and the EmrR mutator gene. Using a fluctuation analysis mutation rate experiment, XL1Blue bacteria transformed with this construct yielded a mutation rate of 3.14E-09 mutations per cell per culture although mutation rate is dependent on growth conditions and can vary depending on experimental setup. This plasmid appears to incur a growth penalty in transformants.
  
 +
Raw data:
 +
 +
[[File:rawdata.jpg]]
 +
 +
Observed mutation count determined by (http://www.keshavsingh.org/protocols/FALCOR.html) by putting in values. Observed mutation count corrected to actual mutation count by the correction factor () as only a portion of each parallel culture was plated.
 +
 
[[File:important_graph.jpg]]
 
[[File:important_graph.jpg]]
 +
 +
The above graph compares mutation rate of wild-type XL1Blue as well as XL1Blue bacteria containing this BioBrick.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 16:52, 1 November 2014

IPTG--GFP--EmrR

Contains an IPTG-sensitive promoter (BBa_J04500), with RBS.3 (BBa_00032), and reporter GFP (E0040) downstream with an additional RBS and the EmrR mutator gene. Using a fluctuation analysis mutation rate experiment, XL1Blue bacteria transformed with this construct yielded a mutation rate of 3.14E-09 mutations per cell per culture although mutation rate is dependent on growth conditions and can vary depending on experimental setup. This plasmid appears to incur a growth penalty in transformants.

Raw data:

Rawdata.jpg

Observed mutation count determined by (http://www.keshavsingh.org/protocols/FALCOR.html) by putting in values. Observed mutation count corrected to actual mutation count by the correction factor () as only a portion of each parallel culture was plated.

Important graph.jpg

The above graph compares mutation rate of wild-type XL1Blue as well as XL1Blue bacteria containing this BioBrick.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1424
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 870