Difference between revisions of "Part:BBa K1419003"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1419003 short</partinfo>
 
<partinfo>BBa_K1419003 short</partinfo>
  
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S32 RNA-OUT component for a universal RNA-IN/OUT regulation system that does not require base pairing to the downstream gene.
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Universal RNA-IN RBS part
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
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Allows for the riboregulation of translation of proteins expresses using this RBS. Based off of the Elowitz RBS (B0034)
  
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Contains the S32 RNA-IN sequence from Mutalik et. al. (2012) spaced appropriately upstream from the RBS. Downstream of the RBS is a start codon and the RFC 25 suffix. When assembled with a protein coding part, there will be an addition of 6 amino acids to the N-terminus. This is necessary to allow for a single RNA-OUT molecule to regulate any protein expressed using this RBS.
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 15:09, 1 November 2014

Universal riboregulatable RBS

Universal RNA-IN RBS part

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 32
  • 1000
    COMPATIBLE WITH RFC[1000]