Difference between revisions of "Part:BBa K777109:Experience"
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==Flic Motility Histogram== | ==Flic Motility Histogram== | ||
https://static.igem.org/mediawiki/2014/f/fc/GU_Figure_2_Motility_histogram.png | https://static.igem.org/mediawiki/2014/f/fc/GU_Figure_2_Motility_histogram.png | ||
| − | <br><b>Figure 2 - FliC Motility Histogram</b> The promoters indicated in the histogram were used to drive the fliC biobrick K1463600 with the RBS B0034. Plasmids containing these constructs were used to complement a chromosomal fliC mutation. The diameter of swimming in a 16 hour swarm assay at 37 degrees is shown. The error bars indicate the range or results obtained in two repeats of the experiment. | + | <br><b>Figure 2 - FliC Motility Histogram</b> The promoters indicated in the histogram were used to drive the fliC biobrick K1463600 with the RBS B0034. Plasmids containing these constructs were used to complement a chromosomal fliC mutation in the stain DS941 delta fliC. The diameter of swimming in a 16 hour swarm assay at 37 degrees is shown. The error bars indicate the range or results obtained in two repeats of the experiment. |
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 14:47, 1 November 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K777109
Team Glasgow 2014 Experience
We made our own version of a fliC biobrick K1463600.
Our version had the MG1655 fliC sequence instead of the DH10B sequence fliC of this biobrick.
Our fliC biobrick restored swimming in a fliC deletion mutant of E. coli.
see BBa_K1463600 for more details.
Flic Motility Histogram
Figure 2 - FliC Motility Histogram The promoters indicated in the histogram were used to drive the fliC biobrick K1463600 with the RBS B0034. Plasmids containing these constructs were used to complement a chromosomal fliC mutation in the stain DS941 delta fliC. The diameter of swimming in a 16 hour swarm assay at 37 degrees is shown. The error bars indicate the range or results obtained in two repeats of the experiment.
User Reviews
UNIQ2bd6e6bbfcfe1f6c-partinfo-00000000-QINU UNIQ2bd6e6bbfcfe1f6c-partinfo-00000001-QINU