Difference between revisions of "Part:BBa K1351033"
Line 10: Line 10:
  
 
This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].
 
This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].
 +
 +
This library was generated utilizing the [https://salis.psu.edu/software/ Salis lab RBS calculator] to find RBS that cover a high space of translation intitiation rate in 'B. subtilis'. It includes seven RBS with the following sequences:
 +
 +
{| class=wikitable
 +
|-
 +
!Number
 +
!BBa_K1310 ..
 +
!Sequence
 +
|-
 +
|1
 +
|28
 +
|aaggagggata
 +
|-
 +
|2
 +
|29
 +
|agaggaggata
 +
|-
 +
|3
 +
|30
 +
|aaggagagata
 +
|-
 +
|4
 +
|31
 +
|aggagaggata
 +
|-
 +
|<b>5</b>
 +
|<b>32</b>
 +
|<b>aagaggagata</b>
 +
|-
 +
|6
 +
|33
 +
|agaaagggata
 +
|-
 +
|7
 +
|34
 +
|aagaagagata
 +
|}
 +
 +
The coverage of RBS strength as calculated by the [https://salis.psu.edu/software/ RBS calculator] is depicted in the Figure 1 shown below. It shows an evenly distributed strength spanning approxiamately three orders of magnitude.
 +
 +
[[File:LMU_14_RBSlibrary.png|thumb|center|400px|Fig.1: Coverage of the translation initiation rate of the RBS library as calculated by the [https://salis.psu.edu/software/ RBS calculator]]]
  
  

Revision as of 06:41, 1 November 2014

LMU Bacillus RBS collection 6

This part was generated in RFC10, and has the following prefix and suffix: 

             Prefix                                                                   Suffix
5' - GAATTC GCGGCCGC T TCTAGA G agaaagggata T ACTAGT A GCGGCCG CTGCAG - 3'
     EcoRI    NotI      XbaI                                         SpeI     NotI   PstI


This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].

This library was generated utilizing the Salis lab RBS calculator to find RBS that cover a high space of translation intitiation rate in 'B. subtilis'. It includes seven RBS with the following sequences:

Number BBa_K1310 .. Sequence
1 28 aaggagggata
2 29 agaggaggata
3 30 aaggagagata
4 31 aggagaggata
5 32 aagaggagata
6 33 agaaagggata
7 34 aagaagagata

The coverage of RBS strength as calculated by the RBS calculator is depicted in the Figure 1 shown below. It shows an evenly distributed strength spanning approxiamately three orders of magnitude.

Fig.1: Coverage of the translation initiation rate of the RBS library as calculated by the RBS calculator


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]