Difference between revisions of "Part:BBa K1363005"
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<p>HfiA is an inhibitor in the holdfast's polysaccharide synthesis pathway. It can inhibit one of the Hfs protein family-HfsJ. So it can block up the synthesis of polysaccharide. HfiA, It is regulated downstream of lovK-lovR and the relationship is as following: [Fig 1]</p> | <p>HfiA is an inhibitor in the holdfast's polysaccharide synthesis pathway. It can inhibit one of the Hfs protein family-HfsJ. So it can block up the synthesis of polysaccharide. HfiA, It is regulated downstream of lovK-lovR and the relationship is as following: [Fig 1]</p> | ||
| − | <figure><img src="https://static.igem.org/mediawiki/parts/6/61/USTC-7.png" width=" | + | <figure><img src="https://static.igem.org/mediawiki/parts/6/61/USTC-7.png" width="400" /><figcaption>Fig 1</figcaption></figure> |
<p>These results support a model in which HfiA inhibits holdfast development via direct interaction with an enzyme required for holdfast bio-synthesis.</p> | <p>These results support a model in which HfiA inhibits holdfast development via direct interaction with an enzyme required for holdfast bio-synthesis.</p> | ||
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<p>In order to let HfiA be regulated by the upstream light sensor system, we add the same promoter as the CI coding domain to its gene. Thus the motion control protein can work with fluorescent proteins in phase. The circuit is as following.[Fig 2]</p> | <p>In order to let HfiA be regulated by the upstream light sensor system, we add the same promoter as the CI coding domain to its gene. Thus the motion control protein can work with fluorescent proteins in phase. The circuit is as following.[Fig 2]</p> | ||
| − | <figure><img src="https://static.igem.org/mediawiki/parts/1/19/USTC-6.png" width=" | + | <figure><img src="https://static.igem.org/mediawiki/parts/1/19/USTC-6.png" width="500" /><figcaption>Fig 2</figcaption></figure> |
<h3>Get this part</h3> | <h3>Get this part</h3> | ||
<p>To get PompC-HfiA, we first PCR the HfiA from genome of C.Crescentus. Then we standardize it and add the promoter to this part. We sequenced it and the result is exactly right. </p> | <p>To get PompC-HfiA, we first PCR the HfiA from genome of C.Crescentus. Then we standardize it and add the promoter to this part. We sequenced it and the result is exactly right. </p> | ||
<figure> | <figure> | ||
| − | <img src="https://static.igem.org/mediawiki/parts/3/3f/USTC-8.png" width=" | + | <img src="https://static.igem.org/mediawiki/parts/3/3f/USTC-8.png" width="400" /> |
<figcaption> PCR result: HfiA together with two another motion control proteins-DgrA and DgrB </figcaption></figure> | <figcaption> PCR result: HfiA together with two another motion control proteins-DgrA and DgrB </figcaption></figure> | ||
Latest revision as of 05:38, 1 November 2014
R0082-HfiA the HfiA protein express passway which can be regulated by light
Introduction
HfiA is an inhibitor in the holdfast's polysaccharide synthesis pathway. It can inhibit one of the Hfs protein family-HfsJ. So it can block up the synthesis of polysaccharide. HfiA, It is regulated downstream of lovK-lovR and the relationship is as following: [Fig 1]
These results support a model in which HfiA inhibits holdfast development via direct interaction with an enzyme required for holdfast bio-synthesis.
These results support a model in which HfiA inhibits holdfast development via direct interaction with an enzyme required for holdfast bio-synthesis.
Circuit
In order to let HfiA be regulated by the upstream light sensor system, we add the same promoter as the CI coding domain to its gene. Thus the motion control protein can work with fluorescent proteins in phase. The circuit is as following.[Fig 2]
Get this part
To get PompC-HfiA, we first PCR the HfiA from genome of C.Crescentus. Then we standardize it and add the promoter to this part. We sequenced it and the result is exactly right.
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]