Difference between revisions of "Part:BBa K1363005"

 
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<partinfo>BBa_K1363005 short</partinfo>
 
<partinfo>BBa_K1363005 short</partinfo>
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<h3>Introduction</h3>
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<p>HfiA is an inhibitor in the holdfast's polysaccharide synthesis pathway. It can inhibit one of the Hfs protein family-HfsJ. So it can block up the synthesis of polysaccharide. HfiA, It is regulated downstream of lovK-lovR and the relationship is as following: [Fig 1]</p>
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<figure><img src="https://static.igem.org/mediawiki/parts/6/61/USTC-7.png" width="300" /><figcaption>Fig 1</figcaption></figure>
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<p>These results support a model in which HfiA inhibits holdfast development via direct interaction with an enzyme required for holdfast bio-synthesis.</p>
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<p>These results support a model in which HfiA inhibits holdfast development via direct interaction with an enzyme required for holdfast bio-synthesis.</p>
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<h3>Circuit</h3>
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<p>In order to let HfiA be regulated by the upstream light sensor system, we add the same promoter as the CI coding domain to its gene. Thus the motion control protein can work with fluorescent proteins in phase. The circuit is as following.[Fig 2]</p>
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<figure><img src="https://static.igem.org/mediawiki/parts/1/19/USTC-6.png" width="300" /><figcaption>Fig 2</figcaption></figure>
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<h3>Get this part</h3>
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<p>To get PompC-HfiA, we first PCR the HfiA from genome of C.Crescentus. Then we standardize it and add the promoter to this part. We sequenced it and the result is exactly right. </p>
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<img src="https://static.igem.org/mediawiki/parts/3/3f/USTC-8.png" width="300" />
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<figcaption> PCR result: HfiA together with two another motion control proteins-DgrA and DgrB </figcaption></figure>
  
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We add an OmpR promoter to the HfiA coding sequence to make it can be regulated by the red and blue upstream photosensitive elements.When there is no light,HfiA expresses and the systhesis of polysaccharose is inhibited.When there is red or blue light,the inhibition is removed and the holdfast is compounded normally.
 
  
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 05:37, 1 November 2014

R0082-HfiA the HfiA protein express passway which can be regulated by light

Introduction

HfiA is an inhibitor in the holdfast's polysaccharide synthesis pathway. It can inhibit one of the Hfs protein family-HfsJ. So it can block up the synthesis of polysaccharide. HfiA, It is regulated downstream of lovK-lovR and the relationship is as following: [Fig 1]

Fig 1

These results support a model in which HfiA inhibits holdfast development via direct interaction with an enzyme required for holdfast bio-synthesis.

These results support a model in which HfiA inhibits holdfast development via direct interaction with an enzyme required for holdfast bio-synthesis.

Circuit

In order to let HfiA be regulated by the upstream light sensor system, we add the same promoter as the CI coding domain to its gene. Thus the motion control protein can work with fluorescent proteins in phase. The circuit is as following.[Fig 2]

Fig 2

Get this part

To get PompC-HfiA, we first PCR the HfiA from genome of C.Crescentus. Then we standardize it and add the promoter to this part. We sequenced it and the result is exactly right.

PCR result: HfiA together with two another motion control proteins-DgrA and DgrB
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]