Difference between revisions of "Part:BBa J45992:Experience"

Revision as of 05:08, 31 October 2006

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Applications of BBa_J45992

J45992 was characterized by measuring flourescence intensity of the in a plate reader. The characterization device was BBa_J45995. In figure A, the absorbance, which is proportional to cell density, is plotted against fluorescence, or roughly the number of GFP molecules present in the culture. It is seen in the graph that J45995 (J45992.E0840) exhibits a much higher level of fluorescence during stationary phase (Absorbance>0.5, see figure B) than during exponential phase (yellow line). Additionally, J45992 was attached to an inverter (BBa_Q04401) and the signal was effectively turned off at stationary phase (green line).

Figure A - J45992 chracterization (yellow), with signal inverted (green), with control constitutive promoter (grey), and with no fluorescent protein coding region (purple)

Figure B - Graph shows that cell reach stationary phase at absorbance ~0.5

User Reviews

UNIQ4cb8fcd5a3c13626-partinfo-00000002-QINU UNIQ4cb8fcd5a3c13626-partinfo-00000003-QINU

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 ===Applications of BBa_J45992===
-J45992 was characterized by measuring flourescence intensity of the in a plate reader.  The characterization device was <bbpart>J45995</bbpart>.  In figure A, the absorbance, which is proportional to cell density, is plotted against fluorescence, or roughly the number of GFP molecules present in the culture.  It is seen in the graph that J45995 (J45992.E0840) exhibits a much higher level of fluorescence during stationary phase (Absorbance>0.5, see figure B) than during exponential phase (yellow line).  Additionally, J45992 was attached to an inverter (<bbpart>BBa_Q04401</bbpart>) and the signal was effectively turned off at stationary phase (green line).
+J45992 was characterized by measuring flourescence intensity of the in a plate reader.  The characterization device was <bbpart>BBa_J45995</bbpart>.  In figure A, the absorbance, which is proportional to cell density, is plotted against fluorescence, or roughly the number of GFP molecules present in the culture.  It is seen in the graph that J45995 (J45992.E0840) exhibits a much higher level of fluorescence during stationary phase (Absorbance>0.5, see figure B) than during exponential phase (yellow line).  Additionally, J45992 was attached to an inverter (<bbpart>BBa_Q04401</bbpart>) and the signal was effectively turned off at stationary phase (green line).
 [[Image:BBa J45992-3characterization.png|left|thumb|600px|<font size=3>Figure A - J45992 chracterization (yellow), with signal inverted (green), with control constitutive promoter (grey), and with no fluorescent protein coding region (purple)</font>]][[Image:osmYgraph.jpg|left|thumb|600px|<font size=3>Figure B - Graph shows that cell reach stationary phase at absorbance ~0.5</font>]]