Difference between revisions of "Part:BBa J36851:Design"

 
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J36851 short</partinfo>
 
<partinfo>BBa_J36851 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
There was originally a XbaI site TCTAGA at nucleotides 118-123 of the part, which was mutated to AGCCGC. It is missing its start codon at the beginning, as the intention was to fuse this with surface display domains upstream.
+
There is no spacer nucleotide between the end of the device and the SpeI restriction site.
  
−
There are no spacer nucleotides between the part and the XbaI restriction site, or between the part and the SpeI restriction site, so that the mixed site would be 6bp long, and reading frame would be maintained between protein domains.  
+
Also, the mixed site in between the parts is only six base pairs long, ACTAGA, without the spacer T or G in between parts. This does not apply between R0010 and B0034 where there are still two spacer nucleotides, and after B0034 where there is still one spacer nucleotide, because R0010 and B0034 are standard bioBrick parts.  
  
  
  
 
===Source===
 
===Source===
−
 
+
Assembly of J04500 and J36847
−
PCR off a plasmid obtained from Alice Ting's lab at MIT.
+
  
 
===References===
 
===References===

Latest revision as of 05:01, 31 October 2006

Lac-inducible generator of Lpp-OmpA(46-159)-Streptavidin single-chain dimeric + His6 tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1458
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1467
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 888
    Illegal AgeI site found at 1422
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is no spacer nucleotide between the end of the device and the SpeI restriction site.

Also, the mixed site in between the parts is only six base pairs long, ACTAGA, without the spacer T or G in between parts. This does not apply between R0010 and B0034 where there are still two spacer nucleotides, and after B0034 where there is still one spacer nucleotide, because R0010 and B0034 are standard bioBrick parts.


Source

Assembly of J04500 and J36847

References