Difference between revisions of "Part:BBa J36850:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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There was originally a XbaI site TCTAGA at nucleotides 118-123 of the part, which was mutated to AGCCGC. It is missing its start codon at the beginning, as the intention was to fuse this with surface display domains upstream.
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There is no spacer nucleotide between the end of the device and the SpeI restriction site.
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+
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There are no spacer nucleotides between the part and the XbaI restriction site, or between the part and the SpeI restriction site, so that the mixed site would be 6bp long, and reading frame would be maintained between protein domains.  
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Also, the mixed site in between the parts is only six base pairs long, ACTAGA, without the spacer T or G in between parts. This does not apply between R0010 and B0034 where there are still two spacer nucleotides, and after B0034 where there is still one spacer nucleotide, because R0010 and B0034 are standard bioBrick parts.
  
 
===Source===
 
===Source===

Revision as of 04:57, 31 October 2006


Lac-inducible generator of Lpp-OmpA(46-159)-Streptavidin wild-type + His6 tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 711
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 753
    Illegal AgeI site found at 804
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is no spacer nucleotide between the end of the device and the SpeI restriction site.

Also, the mixed site in between the parts is only six base pairs long, ACTAGA, without the spacer T or G in between parts. This does not apply between R0010 and B0034 where there are still two spacer nucleotides, and after B0034 where there is still one spacer nucleotide, because R0010 and B0034 are standard bioBrick parts.

Source

PCR off a plasmid obtained from Alice Ting's lab at MIT.

References