Difference between revisions of "Part:BBa K1523023"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1523023 short</partinfo> | <partinfo>BBa_K1523023 short</partinfo> | ||
− | salicylate hydroxylase | + | salicylate hydroxylase from ''Pseudomonas putida'' |
+ | |||
+ | Salicylate + NADH + O2 = catechol + NAD+ + H2O + CO2. | ||
+ | It contains a FAD binding domain. FAD is the cofactor. | ||
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===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 15:59, 31 October 2014
nahG coding sequence
salicylate hydroxylase from Pseudomonas putida
Salicylate + NADH + O2 = catechol + NAD+ + H2O + CO2.
It contains a FAD binding domain. FAD is the cofactor. Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 208
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 208
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 208
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 208
Illegal NgoMIV site found at 120
Illegal NgoMIV site found at 142
Illegal NgoMIV site found at 1042 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1123