Difference between revisions of "Part:BBa K1523022"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1523022 short</partinfo> | <partinfo>BBa_K1523022 short</partinfo> | ||
+ | nahE | ||
The gene encoding trans-o-hydroxybenzylidenepyruvate hydratase-aldolase (tHBP-HA) was isolated from Pseudomonas fluorescens N3, an environmental strain able to degrade naphthalene. | The gene encoding trans-o-hydroxybenzylidenepyruvate hydratase-aldolase (tHBP-HA) was isolated from Pseudomonas fluorescens N3, an environmental strain able to degrade naphthalene. | ||
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+ | Naphthalene is a kind of pollution source. nahE is a member of the naphthalene catabolic in ''Pseudomonas putida'' | ||
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+ | '''Function''' | ||
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+ | Involved in the naphthalene upper catabolic pathway. Catalyzes the transformation of trans-O-hydroxybenzylidenepyruvate (THBPA) to salicylaldehyde and pyruvate. The reaction is reversible. Can also use substrate which carry trans-alpha,beta-unsaturated keto acid side chain and adjacent hydroxyl group such as trans-4-(3-hydroxy-2-thianaphthenyl)-2-oxo-but-3-enoate, trans-4-(3-hydroxy-2-benzofuranyl)-2-oxobut-3-enoate, and trans-4-(3-hydroxy-2-thienyl)-2-oxobut-3-enoate | ||
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+ | [[File:Pathway_Naphthalene.jpg]] | ||
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+ | The F step is ctalyzed by this part K1523022 | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 15:17, 31 October 2014
nahE coding sequence nahE
The gene encoding trans-o-hydroxybenzylidenepyruvate hydratase-aldolase (tHBP-HA) was isolated from Pseudomonas fluorescens N3, an environmental strain able to degrade naphthalene.
Naphthalene is a kind of pollution source. nahE is a member of the naphthalene catabolic in Pseudomonas putida
Function
Involved in the naphthalene upper catabolic pathway. Catalyzes the transformation of trans-O-hydroxybenzylidenepyruvate (THBPA) to salicylaldehyde and pyruvate. The reaction is reversible. Can also use substrate which carry trans-alpha,beta-unsaturated keto acid side chain and adjacent hydroxyl group such as trans-4-(3-hydroxy-2-thianaphthenyl)-2-oxo-but-3-enoate, trans-4-(3-hydroxy-2-benzofuranyl)-2-oxobut-3-enoate, and trans-4-(3-hydroxy-2-thienyl)-2-oxobut-3-enoate
The F step is ctalyzed by this part K1523022
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 631
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 742
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 256
Illegal SapI.rc site found at 148