Difference between revisions of "Part:BBa K1442040"

Revision as of 13:30, 31 October 2014

MS2 bacteriophage coat protein

Regulating the expression of a protein can be achieved through repressive elements; we have the MS2 and MS2 hairpin, which regulate the production of RNA Dependent RNA polymerase (RdRp). As RdRp is fused with MS2 through a P2A linker, an indefinite increase in RdRp production is rate-limited by MS2 production. MS2 is able to bind to the MS2 hairpin sequence, also rate-limiting RdRp production, which in turn regulates the whole replicon system. The iGEM community can benefit from a regulation system, in which MS2 can be fused to a protein of interest to regulate its expression. If the fusion protein is non-functional, engineering restriction sites in the P2A linker and site specific ligation reconstitutes RdRp without the MS2 hairpin. The part derives from Auslander et al, which showed that the MS2 protein-box relationship can be designed for use in synthetic biology to create programmable single-cell mammalian biocomputers with simple expression logic, increasing the complexity of an information processing system without researcher input.

Usage and Biology

The MS2 coat-protein was included in our replicon.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 255
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 255
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 255
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 255
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 255
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 292

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 <partinfo>BBa_K1442040 short</partinfo>
-The MS2 bacteriophage coat protein is a translational repressor [4] that binds the MS2 hairpin to encapsidate the genome and represses replicase synthase translation [1]. Exploiting the MS2 box-protein binding relationship is known as MS2 tagging.
-Isaacs et al [2] noted that the MS2 protein binding box is of use for engineering RNA transcriptional activators via the DNA-protein binding interactions and the hairpin has thus been shown that the MS2 box can be used to drive expression of proteins when localised to a promoter (e.g. the MS2 protein is fused to a DNA-binding protein and the MS2 box is localised to its target promoter, driving expression).
+Regulating the expression of a protein can be achieved through repressive elements; we have the MS2 and MS2 hairpin, which regulate the production of RNA Dependent RNA polymerase (RdRp). As RdRp is fused with MS2 through a P2A linker, an indefinite increase in RdRp production is rate-limited by MS2 production. MS2 is able to bind to the MS2 hairpin sequence, also rate-limiting RdRp production, which in turn regulates the whole replicon system. The iGEM community can benefit from a regulation system, in which MS2 can be fused to a protein of interest to regulate its expression. If the fusion protein is non-functional, engineering restriction sites in the P2A linker and site specific ligation reconstitutes RdRp without the MS2 hairpin. The part derives from Auslander et al, which showed that the MS2 protein-box relationship can be designed for use in synthetic biology to create programmable single-cell mammalian biocomputers with simple expression logic, increasing the complexity of an information processing system without researcher input.
-Furthermore, Auslander et al [3] also showed that the MS2 protein-box relationship can be designed for use in synthetic biology to create programmable single-cell mammalian biocomputers with simple expression logic, increasing the complexity of a informational processing system without researcher input.
 ===Usage and Biology===