Difference between revisions of "Part:BBa K1403003:Design"
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We used LIMS1 CDS from part <partinfo>BBa_I742110</partinfo> and inserted constitutive promoter <partinfo>BBa_J23108</partinfo> and a sRBS upstream of the CDS. Limonene synthase 1 converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent. | We used LIMS1 CDS from part <partinfo>BBa_I742110</partinfo> and inserted constitutive promoter <partinfo>BBa_J23108</partinfo> and a sRBS upstream of the CDS. Limonene synthase 1 converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent. | ||
− | The promoter and RBS were designed as oligos, aligned, digested and ligated. The finished plasmid is in standard | + | The promoter and RBS were designed as oligos, aligned, digested, and ligated. The finished plasmid is in standard biobrick format. |
===Source=== | ===Source=== |
Revision as of 07:11, 31 October 2014
Limonene synthase (LIMS1) expression cassette
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 82
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 187
Design Notes
The purpose of this part is to produce limonene in E. coli.
We used LIMS1 CDS from part BBa_I742110 and inserted constitutive promoter BBa_J23108 and a sRBS upstream of the CDS. Limonene synthase 1 converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent.
The promoter and RBS were designed as oligos, aligned, digested, and ligated. The finished plasmid is in standard biobrick format.
Source
References
Salis Lab RBS Calculator
H.M Salis, Methods in Enzymology 2011
H.M. Salis, E.A. Mirsky, C.A. Voigt, Nat. Biotech., 2009