Difference between revisions of "Part:BBa K1347003"
Line 11: Line 11:
 
<img " src="https://static.igem.org/mediawiki/2014/c/c6/Auxin-and-Coronitine-System-Gif_small_2.gif" />           
 
<img " src="https://static.igem.org/mediawiki/2014/c/c6/Auxin-and-Coronitine-System-Gif_small_2.gif" />           
  
−
<img src=" https://static.igem.org/mediawiki/2014/f/f4/Team_nevada_research_verifying_gfp_in_yeast.png">
+
 
  
 
</html>
 
</html>

Revision as of 02:49, 31 October 2014

COI1

COI1 - COI1 is an E3 ligase that interacts with Jaz proteins (leading to their subsequent ubiquitination and degradation) after coronatin is introduced to the system. It is a system found in plants that is analogous to the auxin-TIR1-AID system studied by the Evry 2012 team.

The 2014 Nevada iGEM team cloned this part into a yeast integrating vector and observed its expression and functionality.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1260
    Illegal BglII site found at 1590
    Illegal BamHI site found at 6
    Illegal XhoI site found at 967
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 826