Difference between revisions of "Part:BBa J45400:Design"
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===Design Notes=== | ===Design Notes=== | ||
Out of time constraints, we did not get rid of the EcoRI and SpeI cut sites in THI3. So...don't use EcoRI or SpeI when making assemblies with this part! | Out of time constraints, we did not get rid of the EcoRI and SpeI cut sites in THI3. So...don't use EcoRI or SpeI when making assemblies with this part! | ||
| − | [[Image: | + | [[Image:IAGDactual.jpg]] |
===Source=== | ===Source=== | ||
Revision as of 04:48, 31 October 2006
3-methylbutanal generator
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1250
Illegal EcoRI site found at 2151
Illegal EcoRI site found at 2850
Illegal EcoRI site found at 3036
Illegal SpeI site found at 1944 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1250
Illegal EcoRI site found at 2151
Illegal EcoRI site found at 2850
Illegal EcoRI site found at 3036
Illegal SpeI site found at 1944 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1250
Illegal EcoRI site found at 2151
Illegal EcoRI site found at 2850
Illegal EcoRI site found at 3036 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1250
Illegal EcoRI site found at 2151
Illegal EcoRI site found at 2850
Illegal EcoRI site found at 3036
Illegal SpeI site found at 1944 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1250
Illegal EcoRI site found at 2151
Illegal EcoRI site found at 2850
Illegal EcoRI site found at 3036
Illegal SpeI site found at 1944
Illegal AgeI site found at 1114 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Out of time constraints, we did not get rid of the EcoRI and SpeI cut sites in THI3. So...don't use EcoRI or SpeI when making assemblies with this part!
Source
The coding regions (BAT2 and THI3) in this part came from S. cerevisae.