Difference between revisions of "Part:BBa K1351022"

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<partinfo>BBa_K1351022 short</partinfo><br>
 
<partinfo>BBa_K1351022 short</partinfo><br>
  
Regulatory rRNA wich binds the binding site sdhC'.  
+
This part was generated with the RFC10 standard and has the following prefix and suffix:
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 +
{|
 +
|prefix with EcoRI, NotI and XbaI:
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|<span style="color:blue">GAATTC</span><span style="color:green">GCGGCCGC</span>T<span style="color:red">TCTAGA</span>G
 +
|-
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|suffix with SpeI, NotI and PstI:
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|T<span style="color:red">ACTAGT</span>A<span style="color:green">GCGGCCG</span><span style="color:blue">CTGCAG</span>
 +
|}
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Sites of restriction enzymes generating compatible overhangs have the same color: <span style="color:blue">EcoRI</span> and <span style="color:blue">PstI</span> in blue, <span style="color:green">NotI</span> in green, <span style="color:red">XbaI</span> and <span style="color:red">SpeI</span> in red.
 +
 
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This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].
 +
 
  
 
Results: [http://2014.igem.org/Team:LMU-Munich/Results LMU-2014-Results]
 
Results: [http://2014.igem.org/Team:LMU-Munich/Results LMU-2014-Results]

Revision as of 18:48, 30 October 2014

FsrA: Fur-regulated sRNA which binds the binding site sdhC

This part was generated with the RFC10 standard and has the following prefix and suffix:

prefix with EcoRI, NotI and XbaI: GAATTCGCGGCCGCTTCTAGAG
suffix with SpeI, NotI and PstI: TACTAGTAGCGGCCGCTGCAG

Sites of restriction enzymes generating compatible overhangs have the same color: EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red.

This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].


Results: [http://2014.igem.org/Team:LMU-Munich/Results LMU-2014-Results]


This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]