Difference between revisions of "Part:BBa K1351022"
Line 2: | Line 2: | ||
<partinfo>BBa_K1351022 short</partinfo><br> | <partinfo>BBa_K1351022 short</partinfo><br> | ||
− | + | This part was generated with the RFC10 standard and has the following prefix and suffix: | |
+ | |||
+ | {| | ||
+ | |prefix with EcoRI, NotI and XbaI: | ||
+ | |<span style="color:blue">GAATTC</span><span style="color:green">GCGGCCGC</span>T<span style="color:red">TCTAGA</span>G | ||
+ | |- | ||
+ | |suffix with SpeI, NotI and PstI: | ||
+ | |T<span style="color:red">ACTAGT</span>A<span style="color:green">GCGGCCG</span><span style="color:blue">CTGCAG</span> | ||
+ | |} | ||
+ | Sites of restriction enzymes generating compatible overhangs have the same color: <span style="color:blue">EcoRI</span> and <span style="color:blue">PstI</span> in blue, <span style="color:green">NotI</span> in green, <span style="color:red">XbaI</span> and <span style="color:red">SpeI</span> in red. | ||
+ | |||
+ | This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus]. | ||
+ | |||
Results: [http://2014.igem.org/Team:LMU-Munich/Results LMU-2014-Results] | Results: [http://2014.igem.org/Team:LMU-Munich/Results LMU-2014-Results] |
Revision as of 18:48, 30 October 2014
FsrA: Fur-regulated sRNA which binds the binding site sdhC
This part was generated with the RFC10 standard and has the following prefix and suffix:
prefix with EcoRI, NotI and XbaI: | GAATTCGCGGCCGCTTCTAGAG |
suffix with SpeI, NotI and PstI: | TACTAGTAGCGGCCGCTGCAG |
Sites of restriction enzymes generating compatible overhangs have the same color: EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red.
This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].
Results: [http://2014.igem.org/Team:LMU-Munich/Results LMU-2014-Results]
This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]