Difference between revisions of "Part:BBa J45100:Design"
(→Source) |
|||
| Line 22: | Line 22: | ||
===Source=== | ===Source=== | ||
| − | Species: Petunia | + | Species: Petunia ''X. hybrida'' from GenBank (AY233465); (also available: ''A. thaliana'' and ''N. suaveolens'') |
===References=== | ===References=== | ||
Revision as of 04:24, 31 October 2006
Wintergreen odor enzyme (BSMT1) generator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 746
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 287
Design Notes
Just CDS taken from GenBank
Designed Forward Strand (1 and 2):
5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G ATG GAA GTT GTT GAA GTT C -3'
- LENGTH: 19
- GC CONTENT: 36.8 %
- MELT TEMP: 47.8 ºC
Designed Reverse Strand (phBSMT1): 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA TTT ATT TTG GTC AAG GAG -3'
- LENGTH: 22
- GC CONTENT: 27.3 %
- MELT TEMP: 46.6 ºC
Source
Species: Petunia X. hybrida from GenBank (AY233465); (also available: A. thaliana and N. suaveolens)
References
<biblio>
- Underwood05 pmid=15849311
- Negre03 pmid=14630969
</biblio>