Difference between revisions of "Part:BBa K1351018"
 
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__NOTOC__
 
__NOTOC__
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This part was generated with the RFC10 standard and has the following prefix and suffix:
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{|
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|prefix with EcoRI, NotI and XbaI:
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|<span style="color:blue">GAATTC</span><span style="color:green">GCGGCCGC</span>T<span style="color:red">TCTAGA</span>G
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|-
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|suffix with SpeI, NotI and PstI:
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|T<span style="color:red">ACTAGT</span>A<span style="color:green">GCGGCCG</span><span style="color:blue">CTGCAG</span>
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|}
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Sites of restriction enzymes generating compatible overhangs have the same color: <span style="color:blue">EcoRI</span> and <span style="color:blue">PstI</span> in blue, <span style="color:green">NotI</span> in green, <span style="color:red">XbaI</span> and <span style="color:red">SpeI</span> in red.
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This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].
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The Promoter regulates the sdpI (immunity against the cannibalism toxin sdpC) and the regulator sdpR in ''Bacillus subtilis''. The overhangs were designed according to RFC10.
 
The Promoter regulates the sdpI (immunity against the cannibalism toxin sdpC) and the regulator sdpR in ''Bacillus subtilis''. The overhangs were designed according to RFC10.

Latest revision as of 16:14, 30 October 2014


This part was generated with the RFC10 standard and has the following prefix and suffix:

prefix with EcoRI, NotI and XbaI: GAATTCGCGGCCGCTTCTAGAG
suffix with SpeI, NotI and PstI: TACTAGTAGCGGCCGCTGCAG

Sites of restriction enzymes generating compatible overhangs have the same color: EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red.

This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].


The Promoter regulates the sdpI (immunity against the cannibalism toxin sdpC) and the regulator sdpR in Bacillus subtilis. The overhangs were designed according to RFC10.
Primer fwd.: GAATTCGCGGCCGCTTCTAGAG gggttgtttttaaaaaaattcaagttataatg
Primer rev.: CTGCAGCGGCCGCTACTAGTA aaaaacatttagacatttagatatttgtataaatg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]