Difference between revisions of "Part:BBa J45992"
Line 6: Line 6:
  
 
===Usage and Biology===
 
===Usage and Biology===
−
This promoter is significantly more active in sationary phase.  Schellhorn, et. al were able to connect the promoter to the LacZ gene in strains grown in rich media.  They were able to show that the beta-Galactosidase activity in stationary phase was 408.8 Miller Units, while the activity was only 8.6 Miller Units in exponential phase.  This preferential activation of the osmY promoter is the result of a complex network of inhibitors and activators which act on the promoter during various stages of the cell cycle.  The promoter is specifically inhibited by H-NS, Lrp, cAMP-CRP, and IHF, while it is specifically activated by the sigma s factor, which is stronly activated by rpos in stationary phase or under starvation conditions (Neidhart 1502-1504).
+
This promoter is significantly more active in sationary phase.  Schellhorn, et. al were able to connect the promoter to the LacZ gene in strains grown in rich media.  They were able to show that the beta-Galactosidase activity in stationary phase was 408.8 Miller Units, while the activity was only 8.6 Miller Units in exponential phase.  This preferential activation of the osmY promoter is the result of a complex network of inhibitors and activators which act on the promoter during various stages of the cell cycle.  The promoter is specifically inhibited by H-NS, Lrp, cAMP-CRP, and IHF, while it is specifically activated by the sigma s factor, which is strongly activated by rpoS in stationary phase or under starvation conditions (Neidhart 1502-1504).
  
  

Revision as of 04:18, 31 October 2006

Full-length stationary phase osmY promoter

This promoter is active in stationary phase and under high osmotic pressure conditions. See experience section for device characterization using J45995 (promoter attached to GFP).


Usage and Biology

This promoter is significantly more active in sationary phase. Schellhorn, et. al were able to connect the promoter to the LacZ gene in strains grown in rich media. They were able to show that the beta-Galactosidase activity in stationary phase was 408.8 Miller Units, while the activity was only 8.6 Miller Units in exponential phase. This preferential activation of the osmY promoter is the result of a complex network of inhibitors and activators which act on the promoter during various stages of the cell cycle. The promoter is specifically inhibited by H-NS, Lrp, cAMP-CRP, and IHF, while it is specifically activated by the sigma s factor, which is strongly activated by rpoS in stationary phase or under starvation conditions (Neidhart 1502-1504).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]