Difference between revisions of "Part:BBa K1413041:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K1413041=== | ===Applications of BBa_K1413041=== | ||
| + | We used OriVR6Kgamma in the pNK2 (BBa_K141344). This plasmid contains a particular origin of replication OriVR6Kgamma. This oriVR6K gamma is controlled by the pi protein, which is encoded by the pir gene. Indeed, the pi protein allows the replication of the plasmid by binding a particular site in the ORI sequence. Hence, the oriVR6K gamma can only be replicated in a bacterial strain producing the pi protein. Also we used the cells DH5alpha pri, which replicated this plasmid. With other bacteria like BL21, or DH5alpha there wasn't replication. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 01:33, 29 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1413041
We used OriVR6Kgamma in the pNK2 (BBa_K141344). This plasmid contains a particular origin of replication OriVR6Kgamma. This oriVR6K gamma is controlled by the pi protein, which is encoded by the pir gene. Indeed, the pi protein allows the replication of the plasmid by binding a particular site in the ORI sequence. Hence, the oriVR6K gamma can only be replicated in a bacterial strain producing the pi protein. Also we used the cells DH5alpha pri, which replicated this plasmid. With other bacteria like BL21, or DH5alpha there wasn't replication.
User Reviews
UNIQ58f59b30176665d7-partinfo-00000000-QINU UNIQ58f59b30176665d7-partinfo-00000001-QINU