Difference between revisions of "Part:BBa K1510233"
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This is a composite of two units, a blue light promoter and a ccdb killing gene. The blue light promoter may turn on the transcription of ccdb.CcdB poisons the gyrase-DNA complex, blocking the passage of polymerases and leading to double-strand breakage of the DNA. Alternatively, in cells that overexpress CcdB, the A subunit of DNA gyrase (GyrA) has been found as an inactive complex with CcdB. The lethal effect of CcdB is most probably due to poisoning of the gyrase-DNA complex. | This is a composite of two units, a blue light promoter and a ccdb killing gene. The blue light promoter may turn on the transcription of ccdb.CcdB poisons the gyrase-DNA complex, blocking the passage of polymerases and leading to double-strand breakage of the DNA. Alternatively, in cells that overexpress CcdB, the A subunit of DNA gyrase (GyrA) has been found as an inactive complex with CcdB. The lethal effect of CcdB is most probably due to poisoning of the gyrase-DNA complex. | ||
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| + | [[file:NYMU_Biosafety picture8.jpg]]Indicated from the above graph, we could discover that after exposure to light for a period of time, the population of E. coli(transformed by ccdb suicide gene) dramatically decrease. And even the population of E. coli reached the stable stage, we can still attain 92.5%proportion of killing. | ||
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| + | [[file:NYMU_Biosafety picture7.jpg]]Inferred from the above graph, we could discover that after exposure to light for a period of time, the survival rate of E. coli(transformed by ccdb suicide gene) dramatically decrease. And the less culture time, less time is needed to kill most of them (In 2hr survival rates are all below 1%). So we could conclude that our modified E. coli could be easily killed by exposure to light in a short upon released. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 21:27, 28 October 2014
A blue light regulated ccdb apoptosis gene
This is a composite of two units, a blue light promoter and a ccdb killing gene. The blue light promoter may turn on the transcription of ccdb.CcdB poisons the gyrase-DNA complex, blocking the passage of polymerases and leading to double-strand breakage of the DNA. Alternatively, in cells that overexpress CcdB, the A subunit of DNA gyrase (GyrA) has been found as an inactive complex with CcdB. The lethal effect of CcdB is most probably due to poisoning of the gyrase-DNA complex.
Indicated from the above graph, we could discover that after exposure to light for a period of time, the population of E. coli(transformed by ccdb suicide gene) dramatically decrease. And even the population of E. coli reached the stable stage, we can still attain 92.5%proportion of killing.
Inferred from the above graph, we could discover that after exposure to light for a period of time, the survival rate of E. coli(transformed by ccdb suicide gene) dramatically decrease. And the less culture time, less time is needed to kill most of them (In 2hr survival rates are all below 1%). So we could conclude that our modified E. coli could be easily killed by exposure to light in a short upon released.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 472