Difference between revisions of "Part:BBa K1510233:Design"

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===Design Notes===
 
===Design Notes===
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Whether the blue light promoter is strong enough to turn on enough ccdb gene, and whether ccdb efficiency greater than cell division ability.
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We use Lactobacillus casei as our chassis in bringing out numeral functions. However, we use E. coli primarily to do our circuit construction, and the testing of its features in biofilm-cleansing and pathogen killing. It is obvious that Lactobacillus casei has less safety concern in contrast to E. coli. Therefore, in afraid of causing environmental danger, we design several safety lock, in the aim of containing harms.
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We constructed a killing gene regulated by a blue light promoter. We picked Ccdb, which is a coding gene that blocks E. coli polymerase to bind on DNA. As for the blue light promoter we uses FixK2 promoter which is regulated by blue light. Both these gene were separately K145151 and K592006 in the iGEM parts registry.
  
 
===Source===
 
===Source===

Latest revision as of 21:06, 28 October 2014


A blue light regulated ccdb apoptosis gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 472


Design Notes

We use Lactobacillus casei as our chassis in bringing out numeral functions. However, we use E. coli primarily to do our circuit construction, and the testing of its features in biofilm-cleansing and pathogen killing. It is obvious that Lactobacillus casei has less safety concern in contrast to E. coli. Therefore, in afraid of causing environmental danger, we design several safety lock, in the aim of containing harms.

We constructed a killing gene regulated by a blue light promoter. We picked Ccdb, which is a coding gene that blocks E. coli polymerase to bind on DNA. As for the blue light promoter we uses FixK2 promoter which is regulated by blue light. Both these gene were separately K145151 and K592006 in the iGEM parts registry.

Source

The blue light promoter was derived from igem kit : K592006. And the ccdb gene was K145151.

References

Bahassi EM1, O'Dea MH, Allali N, Messens J, Gellert M, Couturier M.( 1999 Apr 16) Interactions of CcdB with DNA gyrase. Inactivation of Gyra, poisoning of the gyrase-DNA complex, and the antidote action of CcdA