Difference between revisions of "Part:BBa K1510820"

 
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<partinfo>BBa_K1510820 short</partinfo>
 
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We anneal a RFP at the end of our INPNC display gene, in the hope that under the measure of fluorescence light and O.D value, we can derive pure INPNC expression quantity.  
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We anneal a RFP at the end of our INPNC display gene.We adopted a new method that measuring  fluorescence light and O.D value of our modified e-coli to test the efficiency of INPNC expression. We successfully derived  INPNC expression quantity and expression curve though time. And successfully proved that our targeted gene CSP16 is on the surface of our modified e-coli.  
  
  

Revision as of 20:58, 28 October 2014

RFP indicated INPNC performance evaluation

We anneal a RFP at the end of our INPNC display gene.We adopted a new method that measuring fluorescence light and O.D value of our modified e-coli to test the efficiency of INPNC expression. We successfully derived INPNC expression quantity and expression curve though time. And successfully proved that our targeted gene CSP16 is on the surface of our modified e-coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1036
    Illegal AgeI site found at 2220
    Illegal AgeI site found at 2332
  • 1000
    COMPATIBLE WITH RFC[1000]