|
|
| Line 20: |
Line 20: |
| | <!-- End of the user review template --> | | <!-- End of the user review template --> |
| | <!-- DON'T DELETE --><partinfo>BBa_K1403015 EndReviews</partinfo> | | <!-- DON'T DELETE --><partinfo>BBa_K1403015 EndReviews</partinfo> |
| − | The TMM enzyme is not specific to TMA as a substrate. It is also known to oxidize indole to indoxyl, which dimerizes into the well known blue pigment indigo. Indole is a natural product of tryptophan metabolism in E. coli.
| |
| − | We took advangage of this indole production activity to characterize the TMM enzyme. E. coli that were cultured in LB supplemented with tryptophan (2 g/L) produced a deep blue pigment with absorbance properties matching indigo (Fig. 1). Without TMM expression or without tryptophan, indigo production was minimal or absent.
| |
| − | [[File:Spectrum_indigo.png|center|650px]]
| |
| − | <center>'''Figure 1. Average absorbances of DMSO extractions from bacterial lysates shows TMM activity.'''</center>
| |
| − |
| |
| − |
| |
| − |
| |
| − | GC/MS confirmed the activity of TMM by proving the degradation of trimethylamine (Fig. 2). It was performed on extractions from cultures of TMM-expressing E. coli (TMM) and control expressing an empty vector in a LB medium supplemented with trimethylamine (1 mM). The results show a significant decrease (p-value = 0,0199) of the concentration of TMA in TMM-expressing E.coli (Fig. 5D). This confirms the efficiency of degradation of the fish odor by TMM.
| |
| − | [[File:TMM_GCMS.png|center|800px]]
| |
| − | <center>'''Figure 2. GC/MS analysis of cultures of E. coli expressing TMM or an empty vector (control).'''</center>
| |
Latest revision as of 15:27, 28 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1403015
User Reviews
UNIQaec7f700fb011af0-partinfo-00000000-QINU
UNIQaec7f700fb011af0-partinfo-00000001-QINU
