Difference between revisions of "Part:BBa K1403015"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1403015 short</partinfo> | <partinfo>BBa_K1403015 short</partinfo> | ||
| + | This Biobrick encodes trimethylamine mono-oxygenase (<i>tmm</i>) which transforms trimethylamine into trimethylamin-N-oxide. This enzyme is originally expressed by <i>Ruegeria pomeroyi</i> and is similar to human FMO3. | ||
| + | |||
| + | Trimethylamine + NADPH + H+ + O2 = Trimethylamine-N-oxide + NADP+ + H2O | ||
<ul> | <ul> | ||
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<li>Synthetic RBS</li> | <li>Synthetic RBS</li> | ||
<li>Histidine tag</li> | <li>Histidine tag</li> | ||
| − | + | </ul> | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 15:25, 28 October 2014
Trimethylamine moonoxygenase (tmm) expression cassette This Biobrick encodes trimethylamine mono-oxygenase (tmm) which transforms trimethylamine into trimethylamin-N-oxide. This enzyme is originally expressed by Ruegeria pomeroyi and is similar to human FMO3.
Trimethylamine + NADPH + H+ + O2 = Trimethylamine-N-oxide + NADP+ + H2O
- Constitutive promoter BBa_J23108
- Synthetic RBS
- Histidine tag
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 438
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 262
Illegal AgeI site found at 485 - 1000COMPATIBLE WITH RFC[1000]