Difference between revisions of "Part:BBa K1403002:Design"

(→‎References)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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Codon optimization. Check no restriction sites for EcoRI, SpeI, XbaI and PstI.
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Codon optimized using [http://eu.idtdna.com/CodonOpt IDT online tool]. We checked that there were no restriction sites for EcoRI, SpeI, XbaI and PstI using the software Geneious.
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===Source===
 
===Source===

Revision as of 14:18, 28 October 2014

AgaA expression (3M2H-> 3H3MH)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 288
    Illegal BamHI site found at 519
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 181
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized using [http://eu.idtdna.com/CodonOpt IDT online tool]. We checked that there were no restriction sites for EcoRI, SpeI, XbaI and PstI using the software Geneious.

Source

agaA sequence codon optimized for E. coli 12 (IDT online tool)

References

  • [http://ncbi.nlm.nih.gov/pubmed/12468539 "A specific bacterial aminoacylase cleaves odorant precursors secreted in the human axilla." Acuna G. et al ,2003]