Difference between revisions of "Part:BBa K1403002:Design"
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1403002 short</partinfo> | <partinfo>BBa_K1403002 short</partinfo> | ||
| Line 13: | Line 12: | ||
===Source=== | ===Source=== | ||
| − | agaA sequence codon optimized for E coli 12 (IDT online tool) | + | agaA sequence codon optimized for <i>E. coli 12</i> (IDT online tool) |
===References=== | ===References=== | ||
Revision as of 14:11, 28 October 2014
AgaA expression (3M2H-> 3H3MH)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 288
Illegal BamHI site found at 519 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 181
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimization. Check no restriction sites for EcoRI, SpeI, XbaI and PstI.
Source
agaA sequence codon optimized for E. coli 12 (IDT online tool)