Difference between revisions of "Part:BBa K1333317"

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In the 2014 distribution of biobricks, we found the parts of the promoter: arabinose C (AraC) with Promoter C (PC) (BBa_I13458) and PBad (BBa_I13453) modified by MIT in 2005, and integrated the two parts into araBAD operator (BBa_K1333315). And then, we constructed this araBAD operator before FourU element and RFP.
 
In the 2014 distribution of biobricks, we found the parts of the promoter: arabinose C (AraC) with Promoter C (PC) (BBa_I13458) and PBad (BBa_I13453) modified by MIT in 2005, and integrated the two parts into araBAD operator (BBa_K1333315). And then, we constructed this araBAD operator before FourU element and RFP.
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[[File:IIFR-1-2.jpg|400px|thumb|left|Figure1. The inducible mRFP expression with RNAT-fourU element under PBad.]]  
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[[File:IIFR-1-2.jpg|300px|thumb|center|Figure1. The inducible mRFP expression with RNAT-fourU element under PBad.]]  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 07:43, 28 October 2014

I13458-I13453-FourU-RFP [[File:]]

Usage and Biology

Considering of the different transcription level provided by B2H system, we replace the constitutive promoter(BBa_J23119) in BBa_K1333309 by an inducible promoter, for using different concentration of inducer to simulate this situation. Avoiding destructing the structure of RNAT, we choose the pBAD(BBa_K1333315),induced by L-arabinose, whose operon are in the upstream of promoter.

In the 2014 distribution of biobricks, we found the parts of the promoter: arabinose C (AraC) with Promoter C (PC) (BBa_I13458) and PBad (BBa_I13453) modified by MIT in 2005, and integrated the two parts into araBAD operator (BBa_K1333315). And then, we constructed this araBAD operator before FourU element and RFP.

Figure1. The inducible mRFP expression with RNAT-fourU element under PBad.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1247
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1187
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1873
    Illegal AgeI site found at 1985
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961

Functional Parameters