Difference between revisions of "Part:BBa K1333317"
MolidantaTW (Talk | contribs) (→Usage and Biology) |
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In the 2014 distribution of biobricks, we found the parts of the promoter: arabinose C (AraC) with Promoter C (PC) (BBa_I13458) and PBad (BBa_I13453) modified by MIT in 2005, and integrated the two parts into araBAD operator (BBa_K1333315). And then, we constructed this araBAD operator before FourU element and RFP. | In the 2014 distribution of biobricks, we found the parts of the promoter: arabinose C (AraC) with Promoter C (PC) (BBa_I13458) and PBad (BBa_I13453) modified by MIT in 2005, and integrated the two parts into araBAD operator (BBa_K1333315). And then, we constructed this araBAD operator before FourU element and RFP. | ||
| − | [[File:IIFR-1-2.jpg | + | [[File:IIFR-1-2.jpg|400px|thumb|left|Figure1. The inducible mRFP expression with RNAT-fourU element under PBad.]] |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 07:42, 28 October 2014
I13458-I13453-FourU-RFP [[File:]]
Usage and Biology
Considering of the different transcription level provided by B2H system, we replace the constitutive promoter(BBa_J23119) in BBa_K1333309 by an inducible promoter, for using different concentration of inducer to simulate this situation. Avoiding destructing the structure of RNAT, we choose the pBAD(BBa_K1333315),induced by L-arabinose, whose operon are in the upstream of promoter.
In the 2014 distribution of biobricks, we found the parts of the promoter: arabinose C (AraC) with Promoter C (PC) (BBa_I13458) and PBad (BBa_I13453) modified by MIT in 2005, and integrated the two parts into araBAD operator (BBa_K1333315). And then, we constructed this araBAD operator before FourU element and RFP.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1247
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1187
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
Illegal AgeI site found at 1873
Illegal AgeI site found at 1985 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961