Difference between revisions of "Part:BBa K1333317:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Considering of the different transcription level provided by B2H system, we replace the constitutive promoter(BBa_J23119) in BBa_K1333309 by an inducible promoter, for using different concentration of inducer to simulate this situation. Avoiding destructing the structure of RNAT, we choose the | + | Considering of the different transcription level provided by B2H system, we replace the constitutive promoter(BBa_J23119) in BBa_K1333309 by an inducible promoter, for using different concentration of inducer to simulate this situation. Avoiding destructing the structure of RNAT, we choose the pBAD(BBa_K1333315),induced by L-arabinose, whose operon are in the upstream of promoter. |
In the 2014 distribution of biobricks, we found the parts of the promoter: arabinose C (AraC) with Promoter C (PC) (BBa_I13458) and PBad (BBa_I13453) modified by MIT in 2005, and integrated the two parts into araBAD operator (BBa_K1333315). And then, we constructed this araBAD operator before FourU element and RFP. | In the 2014 distribution of biobricks, we found the parts of the promoter: arabinose C (AraC) with Promoter C (PC) (BBa_I13458) and PBad (BBa_I13453) modified by MIT in 2005, and integrated the two parts into araBAD operator (BBa_K1333315). And then, we constructed this araBAD operator before FourU element and RFP. | ||
Latest revision as of 06:53, 28 October 2014
I13458-I13453-FourU-RFP
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1247
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1187
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
Illegal AgeI site found at 1873
Illegal AgeI site found at 1985 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Design Notes
Considering of the different transcription level provided by B2H system, we replace the constitutive promoter(BBa_J23119) in BBa_K1333309 by an inducible promoter, for using different concentration of inducer to simulate this situation. Avoiding destructing the structure of RNAT, we choose the pBAD(BBa_K1333315),induced by L-arabinose, whose operon are in the upstream of promoter.
In the 2014 distribution of biobricks, we found the parts of the promoter: arabinose C (AraC) with Promoter C (PC) (BBa_I13458) and PBad (BBa_I13453) modified by MIT in 2005, and integrated the two parts into araBAD operator (BBa_K1333315). And then, we constructed this araBAD operator before FourU element and RFP.
Source
The RNAT(FourU) comes from genomic sequence of bacteria.