Difference between revisions of "Part:BBa K1333104"
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NA polymerase III is the main enzyme during DNA replication. DNAQ encodes the epsilon subunit of DNA polymerase III which is responsible for the proofreading function. DNAQ has the 3’-5’ exonuclease activity. During the process of DNA replication ,as soon as base mispairing occur, DNAQ will cut off the newly synthesized base, which enable the right base to substitute its position. This guarantee the accuracy of DNA replication. | NA polymerase III is the main enzyme during DNA replication. DNAQ encodes the epsilon subunit of DNA polymerase III which is responsible for the proofreading function. DNAQ has the 3’-5’ exonuclease activity. During the process of DNA replication ,as soon as base mispairing occur, DNAQ will cut off the newly synthesized base, which enable the right base to substitute its position. This guarantee the accuracy of DNA replication. | ||
DNAQ926 is a mutant of DNAQ, which doesn’t have the proofreading function but still can bind to the polymerase III complex. If DNAQ926 is expressed in the E.coli, it can compete the internal product of DNAQ, which can lead to the mutation during DNA replication. | DNAQ926 is a mutant of DNAQ, which doesn’t have the proofreading function but still can bind to the polymerase III complex. If DNAQ926 is expressed in the E.coli, it can compete the internal product of DNAQ, which can lead to the mutation during DNA replication. | ||
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| + | [[Image:Sysu SOS bypass.jpg|thumb|center|400px|SOS error prone bypass]] | ||
===For more information please visit our wiki=== | ===For more information please visit our wiki=== | ||
Latest revision as of 01:49, 28 October 2014
RBS DNAQ926
The product of DNAQ926 can raise the mutation rate in E.coli.
Usage and Biology
NA polymerase III is the main enzyme during DNA replication. DNAQ encodes the epsilon subunit of DNA polymerase III which is responsible for the proofreading function. DNAQ has the 3’-5’ exonuclease activity. During the process of DNA replication ,as soon as base mispairing occur, DNAQ will cut off the newly synthesized base, which enable the right base to substitute its position. This guarantee the accuracy of DNA replication. DNAQ926 is a mutant of DNAQ, which doesn’t have the proofreading function but still can bind to the polymerase III complex. If DNAQ926 is expressed in the E.coli, it can compete the internal product of DNAQ, which can lead to the mutation during DNA replication.
For more information please visit our wiki
http://2014.igem.org/Team:SYSU-China/content.html#Project/Result/Mutation
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 192
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 64
Illegal AgeI site found at 553 - 1000COMPATIBLE WITH RFC[1000]