Difference between revisions of "Part:BBa K1333102"
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===Usage and Biology=== | ===Usage and Biology=== | ||
When DNA encounters severe damage which may leads to critical problem of the cell, the most prior task is to stabilize the DNA as soon as possible, even at the cost of generate mutation. SOS bypass is an emergency DNA repair method of cells. The functional enzyme of SOS is polymerase V. When the external signal ,like UV, activate the RecA coprotease activity, it can help the lexA gene to cleave itself, which will result in several genes to be induced. UmuD and UmuC are two of the induced genes. The product of UmuD gene will be cleaved by a proteinase and turn into UmuD’. The complex of UmuD’ and UmuC has DNA polymerase activity, which is referred as polymerase V. | When DNA encounters severe damage which may leads to critical problem of the cell, the most prior task is to stabilize the DNA as soon as possible, even at the cost of generate mutation. SOS bypass is an emergency DNA repair method of cells. The functional enzyme of SOS is polymerase V. When the external signal ,like UV, activate the RecA coprotease activity, it can help the lexA gene to cleave itself, which will result in several genes to be induced. UmuD and UmuC are two of the induced genes. The product of UmuD gene will be cleaved by a proteinase and turn into UmuD’. The complex of UmuD’ and UmuC has DNA polymerase activity, which is referred as polymerase V. | ||
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| + | [[Image:Sysu SOS bypass.jpg|thumb|center|400px|SOS error prone bypass]] | ||
===For more information please visit our wiki=== | ===For more information please visit our wiki=== | ||
| − | http://2014.igem.org/Team:SYSU-China/content.html#Project/Result/ | + | http://2014.igem.org/Team:SYSU-China/content.html#Project/Result/Mutagenesis |
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Latest revision as of 01:45, 28 October 2014
RBS umuC
The UmuC is involved in the SOS DNA repair process.
Usage and Biology
When DNA encounters severe damage which may leads to critical problem of the cell, the most prior task is to stabilize the DNA as soon as possible, even at the cost of generate mutation. SOS bypass is an emergency DNA repair method of cells. The functional enzyme of SOS is polymerase V. When the external signal ,like UV, activate the RecA coprotease activity, it can help the lexA gene to cleave itself, which will result in several genes to be induced. UmuD and UmuC are two of the induced genes. The product of UmuD gene will be cleaved by a proteinase and turn into UmuD’. The complex of UmuD’ and UmuC has DNA polymerase activity, which is referred as polymerase V.
For more information please visit our wiki
http://2014.igem.org/Team:SYSU-China/content.html#Project/Result/Mutagenesis
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 195
Illegal BamHI site found at 1202 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 93
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 283