Difference between revisions of "Part:BBa K1383002"

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<partinfo>BBa_K1383002 short</partinfo>
 
<partinfo>BBa_K1383002 short</partinfo>
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===Usage and Biology===
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The ribosome binding sites (RBS) of archaea are not well characterized. By creating and characterizing a library of RBS sequences, researchers will be able to express proteins of interest at variable levels of expression in <i>Methanococcus maripaludis</i>. Ribosome binding sites are typically 6-7 base pair sequences on a transcript that is complementary to the 3’ end of the 16S rRNA. After binding of the RBS to the ribosome, translation will be initiated. An RBS with higher affinity for the ribosome will result in higher rate of translation, and inversely, an RBS with lower affinity will result in lower rate of translation. 
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===Characterization===
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Characterization of the RBS sequences was accomplished using the red fluorescent protein, mCherry as a reporter. Qualitative analysis was evaluated by visualization of the RFP and quantitative analysis was completed through use of a plate reader for reading fluorescence.
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[[Image:Negative-pmev4-mcherry-circuit.png|none|500px|thumb|<b>UGA-Georgia 2014</b> Figure 3: Specific sequence for the 'native' RBS. The region labeled 'Linker' is a few random base pairs that provide the ability to hybridize a gene of interest to the RFP reporter, mCherry. The figure is not drawn to scale.]]
 
[[Image:Negative-pmev4-mcherry-circuit.png|none|500px|thumb|<b>UGA-Georgia 2014</b> Figure 3: Specific sequence for the 'native' RBS. The region labeled 'Linker' is a few random base pairs that provide the ability to hybridize a gene of interest to the RFP reporter, mCherry. The figure is not drawn to scale.]]

Revision as of 01:01, 28 October 2014

BBa_K1383001 (mCherry- Theoretical weakest RBS)

Usage and Biology

The ribosome binding sites (RBS) of archaea are not well characterized. By creating and characterizing a library of RBS sequences, researchers will be able to express proteins of interest at variable levels of expression in Methanococcus maripaludis. Ribosome binding sites are typically 6-7 base pair sequences on a transcript that is complementary to the 3’ end of the 16S rRNA. After binding of the RBS to the ribosome, translation will be initiated. An RBS with higher affinity for the ribosome will result in higher rate of translation, and inversely, an RBS with lower affinity will result in lower rate of translation.

Characterization

Characterization of the RBS sequences was accomplished using the red fluorescent protein, mCherry as a reporter. Qualitative analysis was evaluated by visualization of the RFP and quantitative analysis was completed through use of a plate reader for reading fluorescence.


UGA-Georgia 2014 Figure 3: Specific sequence for the 'native' RBS. The region labeled 'Linker' is a few random base pairs that provide the ability to hybridize a gene of interest to the RFP reporter, mCherry. The figure is not drawn to scale.

In an effort to expand synthetic biology research for Archaea, we have developed protein expression tools to facilitate fluorescence mediated detection of proteins. For our 2014 project we present 3 new BioBrick parts that iGEMers can use readily. All three of our parts are BioBrick compatible. Specifically, we constructed tools consisting of native/ synthetic Methanococcus RBS site(s) upstream of a gene encoding red fluorescent protein-mCherry. Prior to cloning the mCherry gene was codon optimized for expression in Methanoccocus (also, ensuring BioBrick compatibility in the design considerations). The BioBrick part- BBa_K1383002 consists of the theoretical weakest Methanococcus RBS site upstream of the mCherry gene. This fragment was inserted into pSB1C3 plasmid backbone using EcoRI and PstI restriction enzymes.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]