Difference between revisions of "Part:BBa J36849:Design"

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<partinfo>BBa_J36849 short</partinfo>
 
<partinfo>BBa_J36849 short</partinfo>
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===Design Notes===
 
===Design Notes===
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There was originally a XbaI site TCTAGA at nucleotides 118-123 of the part, which was mutated to AGCCGC. It is missing its start codon at the beginning, as the intention was to fuse this with surface display domains upstream.
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There is no spacer nucleotide between the end of the device and the SpeI restriction site.
  
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There are no spacer nucleotides between the part and the XbaI restriction site, or between the part and the SpeI restriction site, so that the mixed site would be 6bp long, and reading frame would be maintained between protein domains.
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Also, the mixed site in between the parts is only six base pairs long, ACTAGA, without the spacer T or G in between parts. This does not apply between R0010 and B0034 where there are still two spacer nucleotides, and after B0034 where there is still one spacer nucleotide, because R0010 and B0034 are standard bioBrick parts.
  
 
===Source===
 
===Source===
  
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PCR off a plasmid obtained from Alice Ting's lab at MIT.
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Assembly of J04500 and J36845
  
 
===References===
 
===References===

Latest revision as of 00:26, 31 October 2006

Lac-inducible generator of Lpp-OmpA(46-66)-Streptavidin single-chain dimeric + His6 tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1179
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1188
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 609
    Illegal AgeI site found at 1143
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is no spacer nucleotide between the end of the device and the SpeI restriction site.

Also, the mixed site in between the parts is only six base pairs long, ACTAGA, without the spacer T or G in between parts. This does not apply between R0010 and B0034 where there are still two spacer nucleotides, and after B0034 where there is still one spacer nucleotide, because R0010 and B0034 are standard bioBrick parts.

Source

Assembly of J04500 and J36845

References