Difference between revisions of "Part:BBa J100201"

Latest revision as of 18:10, 27 October 2014

RBS (BD24) + GFP

This is a composite of superfolder GFP and a BD24 RBS (part BBa J119028). The stop codon of the RBS and start codon of GFP overlap to increase effective translation. This is cloned into pSB1A8 with modified Bba prefix so that there is only an EcoRI site upstream and a normal Bba suffix. We are trying to build indClone for testing inducers. The SNP at base 101 (A--> G) changes the amino acid from V to A. The SNP at base 140 (T --> C) does not change the amino acid.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1
Illegal AgeI site found at 118
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 106

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 This is a composite of superfolder GFP and a BD24 RBS (part BBa J119028). The stop codon of the RBS and start codon of GFP overlap to increase effective translation. This is cloned into pSB1A8 with modified Bba prefix so that there is only an EcoRI site upstream and a normal Bba suffix. We are trying to build indClone for testing inducers.
+The SNP at base 101 (A--> G) changes the amino acid from V to A. The SNP at base 140 (T --> C) does not change the amino acid.
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