Difference between revisions of "Part:BBa K1423006"
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The arsenic inducible promoter converts isoamyl alcohol into isoamyl acetate in the presence of heavy metals, primarily arsenic. This part consists of the Arsenic promoter J33201, Ribosome binding site B0030, ATF1 j45014 (banana odor generator), and a double terminator made up of B0010 and B0012. The generator may express false results, as the generator will produce isoamyl acetate without the presence of arsenic or other heavy metals. But expression appears to rise until a concentration of 5uM arsenite is injected. Any concentrations larger than this appear to be toxic to E.Coli cells, as the production of isoamyl alcohol drops significantly.
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The arsenic inducible banana odor generator consists of the arsenic inducible promoter, J33201, and the composite part J45199, which is made up of a ribosome binding site, the ATF1 gene, and a double terminator. E. coli transformed with this part can convert isoamyl alcohol to isoamyl acetate in the presence of heavy metals such as arsenic and cadmium. The generator may express false results, as the generator will produce isoamyl acetate without the presence of arsenic or other heavy metals. But expression appears to rise until a concentration of 5uM arsenite is injected. Any concentrations larger than this appear to be toxic to E.Coli cells, as the production of isoamyl alcohol drops significantly.
  
 
[https://static.igem.org/mediawiki/2014/b/b6/GCMSresults%282%29.png]
 
[https://static.igem.org/mediawiki/2014/b/b6/GCMSresults%282%29.png]

Revision as of 14:50, 27 October 2014

Arsenic Inducible Banana Odor Generator

WPI_ATF1MetalPromoter.png


The arsenic inducible banana odor generator consists of the arsenic inducible promoter, J33201, and the composite part J45199, which is made up of a ribosome binding site, the ATF1 gene, and a double terminator. E. coli transformed with this part can convert isoamyl alcohol to isoamyl acetate in the presence of heavy metals such as arsenic and cadmium. The generator may express false results, as the generator will produce isoamyl acetate without the presence of arsenic or other heavy metals. But expression appears to rise until a concentration of 5uM arsenite is injected. Any concentrations larger than this appear to be toxic to E.Coli cells, as the production of isoamyl alcohol drops significantly.

[1]

The link above leads to a quantification of the production of isoamyl acetate normalized to the same construct with the high strength constitutive promoter J23100.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 255
    Illegal BglII site found at 646
    Illegal BamHI site found at 1973
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1073
    Illegal SapI.rc site found at 2081