Difference between revisions of "Part:BBa K1333108"

Revision as of 12:09, 26 October 2014

DNAQ926 CDS

DNAQ926 coding sequence,lead to polymeraseIII proofreading defect in E.coli.

Usage and Biology

DNA polymerase III is the main enzyme during DNA replication. DNAQ encodes the epsilon subunit of DNA polymerase III which is responsible for the proofreading function. DNAQ has the 3’-5’ exonuclease activity. During the process of DNA replication ,as soon as base mispairing occur, DNAQ will cut off the newly synthesized base, which enable the right base to substitute its position. This guarantee the accuracy of DNA replication. DNAQ926 is a mutant of DNAQ, which doesn’t have the proofreading function but still can bind to the polymerase III complex. If DNAQ926 is expressed in the E.coli, it can compete the internal product of DNAQ, which can lead to the mutation during DNA replication.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 174
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 46
Illegal AgeI site found at 535
1000
COMPATIBLE WITH RFC[1000]

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 DNAQ926 coding sequence,lead to polymeraseIII proofreading defect in E.coli.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
 DNA polymerase III is the main enzyme during DNA replication. DNAQ encodes the epsilon subunit of DNA polymerase III which is responsible for the proofreading function. DNAQ has the 3’-5’ exonuclease activity. During the process of DNA replication ,as soon as base mispairing occur, DNAQ will cut off the newly synthesized base, which enable the right base to substitute its position. This guarantee the accuracy of DNA replication.